DOSING
Research

Laboratory Protocol

Prep & Injection Guide

A reference sequence for reconstitution and handling under controlled laboratory conditions.

01
Setup

Sanitize Workspace

Wipe the working surface with 70% isopropyl alcohol. Allow to air-dry. Lay out vial, bacteriostatic water, syringes, and alcohol swabs.

02
Inspection

Inspect Lyophilized Vial

Confirm the powder cake is intact and the vial is undamaged. Discard any vial showing discoloration, contamination, or seal breach.

03
Reconstitution

Draw Diluent

Using a 3 mL syringe, draw the calculated volume of bacteriostatic water. Reference the reconstitution chart for your target concentration.

04
Technique

Inject Slowly Down Vial Wall

Angle the needle against the inner wall of the vial. Inject the diluent slowly — never directly onto the lyophilized cake.

05
Handling

Swirl, Do Not Shake

Gently swirl until fully dissolved. Shaking can denature sensitive peptide structures. Allow to settle for 60 seconds.

06
Storage

Store Cold

Label with date and concentration. Refrigerate at 2–8°C. Most reconstituted peptides remain viable 28 days under cold storage.

Reconstitution Chart

Vial
Diluent
Concentration
U-100 Reference
5 mg
2.0 mL
2.5 mg / mL
20 units = 500 mcg
5 mg
2.5 mL
2.0 mg / mL
25 units = 500 mcg
10 mg
2.0 mL
5.0 mg / mL
10 units = 500 mcg
10 mg
3.0 mL
3.33 mg / mL
15 units = 500 mcg

Syringe Selection

0.3 mL / 30u

Single-unit doses (2u, 5u, 9u). Each tick = 1 unit.

30–31g · 5/16" (8mm)

0.5 mL / 50u

Single-unit doses under 10u. Each tick = 1 unit.

30–31g · 5/16" (8mm)

1.0 mL / 100u

Doses over 10 units. Each tick = 2 units.

29–31g · 5/16" or 1/2"

Reconstitution uses a 22–23 gauge, 3 mL syringe. Insulin syringes work in a pinch — use 28–29g, 1/2" tip.

Storage

Reconstituted
Refrigerator · 2–8°C

60–90 days (30–45 for NAD+ / AOD-9604)

Lyophilized
Refrigerator

Up to 1 year

Lyophilized
Freezer

2–3 years · never thaw twice

  • — Opaque, airtight container. Foil-wrap if not opaque.
  • — Add silica desiccant packets to fight moisture.
  • — Keep off the fridge door — agitation degrades peptides.
  • — Never freeze/thaw twice. Aliquot first if storing long-term.
  • — Aliquot in pH 5–6 buffer solution, not BAC water.
  • — Exception: Lipo-C stores at 68–77°F, never refrigerate.

Injection

Subcutaneous
Into Fat

Pinch fat, lift away from body, insert needle fully at 90°. Sites: abdomen (2" from navel), love handles, outer thigh, back of arm.

Intramuscular
Into Muscle

Use 1/2" tip. Push straight through skin into muscle. Sites: deltoid (shoulder), vastus lateralis (outer thigh), ventrogluteal (hip).

  • — Clean vial top and skin with alcohol prep pad.
  • — Draw dose with vial inverted. Inject air first if flow is slow.
  • — Depress plunger fully, wait 2–3 seconds, withdraw.
  • — Rotate sites to avoid lipohypertrophy.
  • — Rub site through shirt for 1 minute to aid absorption.
  • — Cap and dispose in sharps container immediately.