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Research Protocol

NAD+

Important

Read the Prep & Injection Guide for proper reconstitution, syringe sizing, and injection protocols. Mistakes here can compromise your research.

Click here to read the Prep & Injection Guide
01

Pick your vial size

02

Mix with BAC water

For a 500 mg vial, add

5 mL (500 units) BAC water

03

Draw your dose

Period
Units
Dose
Frequency
Week 1
50 units
50 mg
2× per week
Week 2
50–100 units
50–100 mg
2× per week
Weeks 3–6
50–100 units
50–100 mg
4× per week
Weeks 7–8
50–100 units
50–100 mg
4–7× per week
Weeks 9–12
50–100 units
50–100 mg
4–7× per week

Subcutaneous or intramuscular administration. If the dose calls for 100 units, but you would rather take 25, 50, or 75, absolutely do that. We always encourage you to dose conservatively and to only increase your dose as necessary to continue sustaining benefits.

04

Timing & administration

Cycle length

Up to 12 weeks

Followed by a 4–6 week washout period.

Frequency

2–7 days/week

Start at 2× per week and gradually increase frequency as tolerated.

Route

Subcutaneous or Intramuscular

Subcutaneous is slower, steadier, and better tolerated. Intramuscular may cause burning and histamine reactions.

Timing

Morning

Dose in the morning. Not necessary to be fasted; eating a light meal may minimize side effects.

Quick Rules

  • Subcutaneous or intramuscular administration.
  • Up to 12-week cycle followed by 4–6 week washout.
  • Dose in the morning.
  • Not necessary to be fasted; light meal may help side effects.
  • Start at 50 units (50 mg), 2× per week in Week 1.
  • Gradually increase frequency to 4–7× per week by Weeks 7–12.
  • Dose conservatively; 25, 50, or 75 units is fine if 100 feels strong.
  • Rotate injection sites daily for comfort and hygiene.
  • Do not exceed 3,000 mg per month.
  • Do not use while pregnant or breastfeeding.
  • Use caution if diabetic, on blood pressure meds, anticoagulants, or antidepressants.
  • Do not use with active cancer or during cancer treatment.

Do NOT use if

Pregnant or nursing · active cancer or during cancer treatment · avoid stacking with melatonin due to risk of amplified fatigue. Use caution if diabetic (potential insulin resistance), on blood pressure medications (may cause hypotension), anticoagulants such as Coumadin/Warfarin (may interfere with clotting), or antidepressants (may worsen mood-related symptoms). Excessive intake (>3,000 mg/month) may cause liver damage, kidney disease, and increased risk of vascular complications.

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